Effect of Medium and Cytokinin Types on Banana Micropropagation during Multiplication Stage

Document Type : Original Article

Authors

1 Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt.

2 Department of Horticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt

Abstract

Banana (Musa spp. family Musaceae) is a fourth most important fruit crop in the world. This investigation
was carried out in tissue culture laboratory in Horticulture Department, Agriculture Faculty, Suez Canal University,
Ismailia during the period 2013 – 2015, to study the effect of medium type (solid or liquid) and cytokinin types (BAP or
Kin at 0, 1, 3, 5, 7 mg/l) during multiplication stage, in a complete randomized design with two factors. The explants
were collected from suckers grown around banana fruiting mother plants, cultivar Grande Naine. In liquid medium
supplemented with 3.0 mg/l Benzyl amino purine (BAP) increased number of shoots (7.20). While 5 mg/l Kinetin (Kin)
improved shoot length (7.40 cm) in solid medium compared with other treatments. The liquid media is preferable within
mass production and commercial advantages. The BAP promotes shoot initiation and development either with solid or
liquid media more than Kin.

Keywords