Use of Biotechnology for Multiplication of Curcuma longa L. plant during six subcultures

The aim of this study was to set a protocol for the in vitro culture of Curcuma longa L. Sprouted bud
explants of C. longa L. were explanted on Murashige and Skoog (MS) medium supplemented with different
concentrations of 6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1 during six subcultures. The maximum number of
multiplied shoots (8.60 and 8.70 shoots explant-1), was obtained in the medium containing 7 mg l−1 BA at 4th and
5thsubculture with (90 and 100% multiplication frequency) respectively. The optimum BA concentrations for maximum
number of multiplication varied across subcultures. The 7 mg l-1 BA concentration at the late subculture 4th, 5th and 6th
produce highest values of (number of shoots, longest shoot, number of leaves and fresh weight).The maximum number
of roots (6.38 roots explant-1) was induced from 3 mg l−1IBA. The tallest roots (40.6 and 36.5 cm) were obtained on MS
medium supplemented with 1 mg l−1 NAA and 3 mg l−1 IBA. Also, the heaviest fresh weight was obtained from
medium containing 2 and 3 mg l−1 IBA and all concentrations of NAA. In vitro plantlets immediately acclimatized to
greenhouse conditions, showing 100% survival rates in a peat moss and vermiculite (1:3) medium.