Banana (Musa spp. family Musaceae) is a fourth most important fruit crop in the world. This investigationwas carried out in tissue culture laboratory in Horticulture Department, Agriculture Faculty, Suez Canal University,Ismailia during the period 2013 – 2015, to study the effect of medium type (solid or liquid) and cytokinin types (BAP orKin at 0, 1, 3, 5, 7 mg/l) during multiplication stage, in a complete randomized design with two factors. The explantswere collected from suckers grown around banana fruiting mother plants, cultivar Grande Naine. In liquid mediumsupplemented with 3.0 mg/l Benzyl amino purine (BAP) increased number of shoots (7.20). While 5 mg/l Kinetin (Kin)improved shoot length (7.40 cm) in solid medium compared with other treatments. The liquid media is preferable withinmass production and commercial advantages. The BAP promotes shoot initiation and development either with solid orliquid media more than Kin.