Effect of Media Type and BAP Concentrations on Micropropagation during Multiplication Stage on Ponytail Palm (Beaucarnea recurvata Lem.)

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Abstract

Abstract: Ponytail palm (Beaucarnea recurvet Lem.; Family Asparagaceae) is one of the most important plants in the
internal and external coordination. This work was carried out to study the effect of media type (MS, B5 and WPM) and
Benzylaminopurine BAP at 0, 0.2, 0.4 and 0.6 mg/l during multiplication stage. The shoot tips were collected from in
vitro seedlings cultured on MS medium without growth regulators. B5 medium supplemented with 0.4 mg/l (BAP)
increased number of shoots (3.40 shoots/clump) and number of leaves (24) plant compared with other treatments. The
B5 medium is preferable within mass production and featured commercial. The BAP Concentration 0.4 mg/l promotes
shoots initiation and development with B5 medium more than MS and WPM.

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