Scientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Effect of Rootstocks on Vegetative Growth, Yield and Fruit Quality of Cucumber11058503ENJournal Article20190205Effectiveness of grafting cucumber cultivars ‘Kattia’ and ‘Hesham’ onto Ferro and ‘Shintoza’ as rootstocks on growth and yield was evaluated during two successive seasons 2015/16 and 16/17 under plastic house. Stem length, both No. of leaves and fruits plant-1 were periodically measured after 30, 45, 60 and 90 days from transplanting. At harvest, in 130-days old plants, both No. of branches and nodes, stem diameter(cm) and leaf area (cm2), yield (kg plant-1 and kg m-2); fruit quality as dry weight (g), water content (%), length (cm),diameter (mm) and SSC (%) of fruits were estimated. Concentrations of N, P and K in leaves and roots were also determined. Results cleared that ‘Kattia’ cv. plants were more affected by grafting for vegetative parameters such as stem length, No. of leaves, branches and nodes as well as stem diameter more than ‘Hesham’ cv. Enhancement of vegetative growth was paralleled with high K content in roots. Grafting delayed the fruiting in both cultivars. Grafting decreased both SSC% and water content (%) in fruits in both cultivars. ‘Hesham’ cv. had the highest leaf area and yield plant-1 with high concentration of N in leaves and roots as well as P in roots. Yield increased by 70.7 and 67.6% in ‘Hesham’ cv. after grafting on Ferro rootstock in both seasons, respectively. ‘Hesham’ cv. was more compatible with Ferro rootstock than ‘Shintoza’, due to earlier formation of adhering layer and callus as well as xylem vessels differentiation. It can be concluded that, Ferro is suitable rootstock for cucumber grafting to increase the yield and improve quality of fruits.https://hjsc.journals.ekb.eg/article_58503_1f81586d6913f938148c5c4cf53ad9db.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Effect of UV-C Radiation on Egyptian Henbane (Hyoscyamus muticus L.) Callus Growth and Biochemical Components11215984510.21608/hjsc.2019.59845ENAbdelrazik E. M. A. M. El-hamahmy; I. Abo-Elsoud; Elham M. AliAkram A. AboseidahJournal Article20190217Physiological impact of different exposure periods (1, 2 and 3 h) of UV-C (253.7 nm) on alkaloids and hyoscyamine production in 21-days aged callus of Egyptian henbane was investigated. Results showed that the concentration of total alkaloids was increased by 2.7 and 2.3 times after exposure to UV-C for 2 or 3 h, respectively more than unstressed callus. Also, data of HPTLC revealed that 2 h exposed-callus accumulated the highest amount of hyoscyamine which similar to the amount in wild leaves but it was more 3.1 times than control. Increment of alkaloids concentration was attributed with high accumulation of non-enzymatic antioxidants as free amino acids, free phenolics and glutathione and increased of peroxidase and superoxide dismutase activity as well as reduction of H<sub>2</sub>O<sub>2 </sub>concentration. Also, 2 h exposed-callus to UV-C showed high expression of electrophoretic protein bands with molecular weights 109, 42 and 29 KDa. All exposure times to UV-C increased both fresh and dry weight of callus compared to unstressed one. Short exposure time of UV-C enhanced the amount of Chl. a and b in callus than other treatments. All exposure periods of UV-C decreased the concentration of carotenoids, anthocyanine, proline and total carbohydrates than control. Long exposure period of UV-C modified the callus cells shape by induction large spherical cells and lysigenous intercellular spaces. It can be concluded that 2 h exposure time of UV-C is effective treatment for enhancement both alkaloids and hyoscyamine content in callus of Egyptian henbane.https://hjsc.journals.ekb.eg/article_59845_6d4c2d18a4f586f17ff3617ab29af93c.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Thidiazuron and Explant Type Effects on High-frequency In Vitro Mass Propagation of Cherry Tomato23335984610.21608/hjsc.2019.59846ENGenesiaFarouk OmarJournal Article20190310Cherry Tomato is one of the most important vegetable crops cultivated for export in Egypt. <em>In vitro</em> culture response was assessed in tomato (<em>Solanum lycopersicum L. var. cerasiforme</em>) cv. (Summer Cherry) for optimum callus induction and plantlet regeneration. Callus induction was achieved within eight to 12 days directly on the cut surfaces of hypocotyl, cotyledon and leaf disc explants cultured on Murashige and Skoog (MS) basal medium supplemented with various concentrations of Thidiazuron (TDZ)] and benzyl adenine (BA) alone, but not in hormone free-medium. The highest callusing index (3.9 and 3.7) was obtained on hypocotyl explants cultured on MS medium supplemented with TDZ(1.0 and 2.0 mgl<sup>-1</sup>) followed by an index of 3.5 obtained from the same explant by using 0.5mgl<sup>-1</sup> BA. However, for the leaf disc explants, the highest callusing index (3.1) was obtained on MS medium supplemented with BAat 2.0 mgl<sup>-1</sup>. After 8 weeks of culture, organogenesis was observed only on the explants cultured on medium containing different concentrations of TDZ and BA. The best shoot formation (93%) was obtained from leaf disc explant callus induced on MS medium containing TDZ. The highest number (13.4) of shootsexplant<sup>-1</sup> was found when cotyledon explant callus was sub cultured on MS medium supplemented with 2.0 mgl<sup>-1</sup> TDZ. Half strength of MS was found to be the best rooting medium, however, addition of IAA at 1.0 mgl<sup>-1</sup> and IBA at 2.0 mgl<sup>-1</sup> were found necessity to induce highest number of roots (22.5) and longer roots (11.0 cm), respectively. Acclimation of <em>in vitro</em> rooted plant is important for testing the post culture behavior of tissue culture regenerated plants. Cherry tomato derived from different explant sources under different concentration of TDZ and BA were not significantly different in their vegetative characters to those obtained from seed. However number of (raceme plant<sup>-1</sup>, flower raceme<sup>-1</sup>, fruit raceme<sup>-1</sup>, fruit plant<sup>-1</sup>) which produced by seed-derived plants was significantly less than those derived from <em>in vitro</em> propagated plants. This protocol would be valuable to create somaclonal variation and develop transgenic approaches for varietal improvement of cherry tomato.https://hjsc.journals.ekb.eg/article_59846_9770f15234f1a15ad8ac840b4df1f420.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Growth and Yield Response of Garlic Genotypes to Foliar Application of γ-Aminobutyric Acid35435984810.21608/hjsc.2019.59848ENEltohamy A. A. YousefIbrahim N. NasefJournal Article20190107Worldwide, garlic is one of the most important bulb vegetable crops and it has been cultivated from thousands of years ago due to its medicinal and nutritional benefits. The main goal of the current research was to explore the effect foliar application of <strong>γ-</strong>aminobutyric acid (GABA) on vegetative development and yield traits as well as chemical constitutes of garlic. To achieve this goal, two field experiments were performed during winter seasons of 2015/2016 and 2016/2017. Four concentrations of GABA (0, 1, 2 and 4 mM) were sprayed on two garlic's genotypes (Sids 40 and Egaseed). The results reported that exogenous supplementation of GABA notably improved vegetative growth (plant height and plant fresh weight), yield traits (bulb weight and total yield), and minerals content (N, P, K and Ca), as well as the content of organic compounds (total chlorophyll and total phenols) compared to non-treated plants (control) in both genotypes. It was clearly revealed that 1 mM GABA treatment had achieved the highest morphological traits, yield traits, chemical composition and organic compound content compared to the other GABA treatments. The findings indicate that GABA application has the ability to enhance growth and productivity of garlic through enhancing mineral composition and organic compounds.https://hjsc.journals.ekb.eg/article_59848_bced43d3f8d607cd49953e504dc5be3a.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Early potato production is positively affected by potassium fertilization45545984910.21608/hjsc.2019.59849ENIbrahim N. NasefEltohamy A. A. YousefJournal Article20190217Two field experiments were conducted during 2017-2018 and 2018-2019, at the Experimental Farm, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt with aim of investigation of potassium application on earliness of potato. To achieve this goal, five different net potassium levels: 0, 60, 90, 120, and 150 kg/Feddan were applied and potato tubers were harvested at two harvesting times: 90 and 105 days after planation. The results reported that both potassium application and harvesting time as well as their interaction significantly affected most of the studied traits. The highest values of plant height, plant fresh weight, plant dry matter, tuber dry matter, marketable yield/feddan, total yield/feddan, soluble solid content (SSC), nitrogen (N) content and potassium (K) content were obtained from the plants received 120 kg K and harvested at 105 days after planation. While, the potassium fertilized plant at 120 kg/feddan and harvested at 90 days achieved the maximum values of total yield per plant, total sugar and non-reducing sugars. However, the difference between these two treatments was not necessarily significant for some traits. In addition, the results of the current study interestingly clarified that potassium level (150 kg) negatively affected some yield attributes in both harvesting times compared to the other potassium levels. Consequently, the result of the current study indicates that high level of potassium (120 kg) can hasten the earliness of potato, which may achieve notable financial profits for potato growers.https://hjsc.journals.ekb.eg/article_59849_ed7bd65be751e715a902156e5555ea6c.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Phenotypic, Biochemical and Molecular Characterization of New Egyptian Mango Genotypes55685985210.21608/hjsc.2019.59852ENNaglaa K. H. Serry Mohamed S. M. AliMohammed A. A. HusseinJournal Article20190415The first step towards improving mango production was started by the selection of eleven genotypes because of their superior horticultural characteristics and fruit quality parameters, from Suez Canal University orchard through 2016 to 2018 seasons. All studied mango genotypes (G) differed in dates of full bloom, maturity stage, bearing habit, floral malformation and fruit characteristics. All genotypes had commercially acceptable qualities and produced relatively high yield ranged from 298.7 - 394 Kg/tree. Generally, genotype No. 9 recorded the highest value (96.6%) in the general evaluation according to yield, Biennial bearing and fruit quality, followed by genotypes No. 7 and No. 5 which scored 94.0 and 91.8%, respectively. In general, these three genotypes possessed most of the desired commercial attributes of international standard. In addition, further understanding of the structure of genetic diversity was done using nine SSR markers based on their scorability, repeatability and capability to differentiate among studied genotypes. Also, analyze of DNA fingerprinting data to create molecular IDs was done to get an idea for the conservation and protection of studied genotypes. The nine SSR loci produced a total of 32 alleles with 97% polymorphism and observed alleles per locus diversified from 1 to 8 alleles with an average of 3.6 without zero alleles. The observed heterozygosity (Ho) within the loci ranged from 0.0 to 0.55 with an average value of 0.23, heterozygosity level within the genotypes varied from 0.11 to 0.44. The polymorphic information content (PIC) values ranged from 0.0 to 0.86 with an average value of 0.49, whereas the mean of discriminating power (DP) for all loci was 0.51. Regarding the probability of matching fingerprints, none of the genotypes were different or similar at all loci with any of the rest genotypes and this value ranged from low to medium with an average of 0.48. The similarity degree ranged from 0.18 to 0.90 with an average of 0.53, indicating that the genetic differences were moderate among mango genotypes under study. Whereas, seven of these genotypes produced 11 unique bands maybe accompanying to number of novel functional alleles, which can be used in mango improvement.https://hjsc.journals.ekb.eg/article_59852_c07618dfee8dcb82d4752c9f381fc627.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Effect of some Biological Stimulants and Kaolin Particles Sprays on Fruit Retention, Productivity and Fruit Quality of Washington Navel Orange Trees69785985510.21608/hjsc.2019.59855ENMohamed S. M. AliHamdy E. El ZayatJournal Article20190421This investigation was performed out during seasons 2016 and 2017 at private grove in El-Salhia El-Gadida, Sharkia Governorate, Egypt on 11 year-old Washington navel orange trees are growing in sandy soil under drip irrigation system. The effects of spraying salicylic acid (SA) (200 and 400 mg/L), Jasmine oil (JO) (2 and 4 ml/L), radish root extract (RRE) (50 and 100 ml/L) and Kaolin particles film (KPF) (5000 and 10000 mg/L) were studied on vegetative growth (leaf area index and leaf chlorophyll value), fruit set, fruit retention, productivity (yield as number and weight per tree), fruit quality parameters (fruit weight, fruit diameter, secondary fruit diameter, peel thickness, firmness, juice content, TSS, TA, TSS/TA ratio, ascorbic acid) and nutritional status (N, P, K and Ca contents). The current study clearly demonstrated that SA at all concentrations especially at 200 mg/L improved leaf area index, fruit retention, fruit number per tree, fruit yield, fruit firmness, juice content percentage, TSS, ascorbic acid and decreased the total acidity in orange juice, as well as increased nutritional status (N and P),. Also, a positive response in fruit retention, fruit weight, peel thickness, leaf area, chlorophyll value and N and P contents by foliar application of KPF at all concentrations under study. Moreover, Foliar sprays with JO at 2 ml/l and RRE at 100 ml/l significantly increased fruit set, fruit weight, fruit diameter, peel thickness, and lowered acidity percentage and increased Ca content.https://hjsc.journals.ekb.eg/article_59855_aa9c1722a19447dd105a45188e853d4b.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Using Boron, Magnesium and some Amino Acids to Improve Yield and Fruit Quality of Roomy Red Grapevines79866641910.21608/hjsc.2019.66419ENMostafa A.A. Mohamed,El-Sayed M. QaoudJournal Article20191210This study was carried out during 2017 and 2018 seasons to study the effect of boric acid, Magnesium sulphate and some amino acids (Tryptophan, Methionine and Arginine) at 0.05%, 0.2% and 0.1% respectively, either alone or combination on solving clusters looseness, shot berries and coloration problems in Roomy Red grapevines grown under Minia region. Vegetative growth, vine nutritional status, berry setting %, yield, cluster weight as well as physical and chemical characteristics of the berries were positively affected by using amino acids, magnesium sulphate and boric acid either as a single or in combinations when compared to the check treatment. Also, shot berries % and total acidity reduced greatly with application of such treatments. Using boric acid, magnesium sulphate and amino acids influenced positively the studied characters in ascending order. Combined applications showed more effects rather than single ones in this respect. Three sprays of a mixture containing boric acid, Magnesium sulphate and some amino acids (Tryptophan, Methionine and Arginine) at 0.05%, 0.2% and 0.1%, respectively, was responsible for obtaining higher yield, lower shot berries and clusters looseness as well as promoting berries quality of Roomy Red grapevines.https://hjsc.journals.ekb.eg/article_66419_174ea88938f121d176d2e909567f04aa.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Effect of some Plant Extracts and Oils on Yield and Fruit Quality of Naomi Mango Cultivar87946850910.21608/hjsc.2019.68509ENEl-Sayed MostafaQaoudHorticultural Department, Faculty of Agriculture, Suez Canal University0000-0002-3514-0412Journal Article20191201Five plant extracts and oils namely green tea extract, turmeric extract, black seed oil, onion oil and garlic oil each at 2% were tested for evaluating their effect on yield and fruit quality of the Naomi Mango trees, during 2017-2018 seasons, these extracts were sprayed two times, one at full bloom and second at pea stage. The plant extract improve all physical and chemical characters, respectively. Turmeric extract, onion oil and garlic oil were more effective to improve tree yield, fruit shape, firmness, gave the best red color and decreased the enzyme activities. Green tea extract increased vitamin C and total phenols. Black seed oil increased fruit acidity percentage and showed the lowest ratio of TSS/ TA. Generally, foliar sprays of plant extracts and oils improved fruit quality and help in delay fruit ripening, which is important in fruit marketing.https://hjsc.journals.ekb.eg/article_68509_b0937f17dc076fbb9aca3c39cbdfdc00.pdfScientific Society of Agricultural SciencesHortscience Journal of Suez Canal University2314-79468120191201Effect of Spraying Fish Oil and Glutathione on Fruiting of Ewaise Mango Trees Grown under Sandy Soil951086970110.21608/hjsc.2019.69701ENHamdySaiedJournal Article20191210This study was established during 2017 and 2018 seasons to test the effect of single and combined applications of fish oil and glutathione each at 0.5 to 2% on some vegetative growth aspects, photosynthetic pigments, flowering and fruit setting behavior, N, P, K, Mg, Ca (as %), Zn, Fe, Mn and Cu (as ppm), yield and both physical and chemical characteristics of the fruits in mango cv. Ewaise grown under sandy soil. The trees received three sprays at growth start, just after fruit setting and at 30 days later. Single and combined applications of fish oil and glutathione each at 0.5 to 2% had substantial promotion on shoot length and thickness, number of leaves/shoot, leaf area (in the spring growth cycle), photosynthetic pigments (chlorophylls a & b, total chlorophylls and total carotenoids), N, P, K, Mg, Ca (as %), Zn, Fe, Mn and Cu (as ppm), panicle length, number of panicles/tree, perfect flowers %, percentages of initial and fruit retention, yield and fruit quality characteristics over the control. Using glutathione was reasonably superior than using fish oil in enhancing all the studied parameters. Companied applications were materially favourable than using each material alone. In all cases negligible effect was observed on these parameters among the higher two concentrations of each material (1 & 2%). The best results with regard to growth, tree, nutritional status, flowering, fruit setting, yield and fruit quality were obtained due to treating Ewaise mango trees grown under sandy soil thrice (growth start, just after setting and at one month later) with a mixture of fish oil and glutathione each at 1%.https://hjsc.journals.ekb.eg/article_69701_464ed33a3b787f8a642b3e20e77bb3b1.pdf