ORIGINAL_ARTICLE
Physiological Effect of Potato Genotypes and Salicylic Acid on Plantlets Growth and Microtuber Production under Salt Stress
This study was conducted to investigate the effectiveness of SA (10 and 100 μmol) for induction of salinity tolerance of two potato genotypes “Proventa” and line “97-980” during vegetative and micro-tuberization stages. Results showed that decreasing of plantlet weight and length as well as microtuber weight under saline conditions differed according to genotypes and SA concentration. Although line “97-980” had low percentage of decreasing in weight and length of plantlets compared to “Proventa” under saline condition, Proventa cv. Gave high microtubers weight. Line 97-980 recorded high reducing sugars, phenolics, proline, amino acids and proteins as well as high activity of peroxidase (POD). On contrary, “Proventa” had high concentration of total chlorophylls and high activity of superoxide dismutase(SOD) and catalase (CAT).The decrease in plantlets growth was lower in line 97-980 compared to” Proventa” under low concentration of SA. Microtubers weight in “Proventa” was higher with addition of 10 μmol through increment in most investigated biochemical compounds and high antioxidants enzymes activity. It may be concluded that high content of reducing sugars, phenolics, proline, amino acids, proteins as well as high activity of POD could work as selectable markers for in vitro potato tolerance to salinity. Addition of low concentration of SA had beneficial effect on physiological
https://hjsc.journals.ekb.eg/article_59092_c0a27fb5badf43a747fad7899632e737.pdf
2018-08-20
7
14
10.21608/hjsc.2018.59092
Solanum tuberosum L
Biochemical compounds
antioxidants activity
salt tolerance
microprpagation
Elwan M. W. M.
F. H. Mohamed
1
AUTHOR
ORIGINAL_ARTICLE
Plant growth, root yield and nutrients content of carrot as affected by the source of nitrogen and application of micronutrients
This study was carried out to give some information that could improve imported cultivars of carrot for theirnutritional status and production. In this work the effect of two N forms (ammonium nitrate and ammonium sulphate)and two levels of micronutrients, without and with (FeEDTA+MnEDTA +MnEDTA) using two imported cultivars(Nants and Kuroda) on the growth, yield and macro and micronutrients in shoots as well as in roots were studied. Theresults indicated that the plant growth and yield were affected by the source of N and application of micronutrients,whereas generally the best results were obtained with ammonium sulphate and foliar application of micronutrients. Theresponse of imported cultivars was quite similar. Macro and micronutrients in shoots as well as in roots were slightlyaffected by the genotypes, N-forms and spraying of micronutrients with unclear trend
https://hjsc.journals.ekb.eg/article_59094_da9df43685857988d0cad878eeb87edb.pdf
2018-07-20
15
24
10.21608/hjsc.2018.59094
Daucus carota
N-fertilizer
macro and micronutrients
yield
Elwan, M. W. M
M. S. S. Soliman
1
AUTHOR
ORIGINAL_ARTICLE
Response of Watermelon Plants Grafted onto Different Cucurbit Rootstocks to Sub-Optimal Growing Temperature
This study was conducted to examine the relative tolerance of grafted watermelon to sub-optimal growing temperature conditions. Watermelon cv. Aswan scions were grafted onto different rootstocks, namely Giada, Shintoza, StrongToza, and Ferro. Grafted and un-grafted seedlings were grown in the field under low temperature during Jan.-Apr. to be compared to those growing under normal warm season conditions (Mar.-Jun.). Results indicated significant decrease in growth, yield, chlorophyll, and carotenoids in all grafted plants, in different degrees, depending on the graft combination. In this regards, plants of Aswan/Ferro recorded more than double the marketable yield of un-grafted ones under sub-optimal temperature, indicating the relative tolerance of Aswan/Ferro to cold stress. While, un-grafted plants exhibited the highest decline in all growth and yield parameters. Leaf proline content and the activities of anti-oxidant enzymes (SOD and CAT) were higher under cold stress than the control. It is concluded that grafted watermelon plants were relatively tolerant to sub-optimal temperature than un-grafted ones which could enable the production of out of season crop.
https://hjsc.journals.ekb.eg/article_59095_93de2093c6d34b9409bb4dea857aa187.pdf
2018-07-20
25
34
10.21608/hjsc.2018.59095
Citrullus lanatus
grafting
rootstocks
cold stress
iso-enzymes
ORIGINAL_ARTICLE
Runner Production of Strawberry Plants in Soilless Suspended System: Nitrogen Rate, GA3 and Genotype Effects
Two separate experiments were conducted at the greenhouse facilities of the Department of Horticulture,Faculty of Agriculture, Suez Canal University during the period of 2014-2016 with the objective of optimizingstrawberry runner and ramet production on soilless, suspended growing system. The first experiment aimed to test the effect of nitrogen fertilizer rates (500 vs. 1000 ppm), GA3sprays and their combinations. Results indicated that fertigation with high N rate in combination with GA3 (50 ppm) spraying on mother plants resulted in higher runner number (37.7%), ramet number (45%) and runner length (19%) than the control. Fertigation with high N alone significantly increased mother plants crown diameter, crown FW and DW, number of leaves and leaf area over all other treatments. Application of N at 1000 ppm + GA3 spraying increased N, P, Mg, Ca, Fe and Zn in mother plant leaves. The second experiment was conducted to evaluate different strawberry genotypes ('Festival', 'Tudla', 'Sweet Charlie', 'Gaviota' and 'Camarosa') for their runnering behavior in suspended growing system and the results indicated that cv. "Festival" produced higher runner and ramet number per mother plant, followed by cv. 'Tudla' compared to the other cultivars, while ramets FW was higher in cv. 'Gaviota' and 'Sweet Charlie' than 'Festival', 'Tudla' and 'Camarosa'. Mother plants of cv. 'Festival' recorded the highest crown diameter, crown FW and DW, followed by 'Gaviota', while cv. 'Sweet Charlie' recorded higher root length and root FW. The highest photosynthetic pigment contents were recorded in cv. 'Tudla', followed by 'Festival'. Nutrient analysis of leaves indicated that the highest N, Fe, Mn, Zn cud Cu were in leaves of cv. 'Festival', while 'Camarosa' recorded the highest P, and 'Sweet Charlie' had the highest K and Ca. RAPD-PCR analysis of DNA of the used strawberry cultivars indicated their different genetic makeup, with about 20% polymorphism using three selected primers which may explain their differences in runner production.
https://hjsc.journals.ekb.eg/article_59097_46b4c3663a067d4eccac8804389ed300.pdf
2018-07-20
35
46
10.21608/hjsc.2018.59097
Fragaria x ananassa Duch
stolon (runner)
ramet (daughter plant)
nitrogen
GA3
genotypes
RAPD-PCR
Mohamed, F.H
A. A. El-Deeb
1
AUTHOR
ORIGINAL_ARTICLE
Effect of Some Growth-Stimulants on Growth, Yield and Storability of Onion Plants (Allium cepa L.)
Improving growth, quality and increasing yield of onion plants are important goals for growers torealize the requirements of the market and the consumers. Two field experiments were carried out during twosuccessive seasons; 2013/2014 and 2014/2015 at a private farm in El-Mahalla El-Kubra district, El- GharbiaGovernorate, Egypt to find out the influence of some growth- stimulants at different rates viz., yeast extract (2, 3and 4gl-1 ) seaweed extract (3,4 and 5 gl-1) and NAA (0.8, 1.2 and 1.6 gl-1) on vegetative, yield, and its components as wellas bulb storability of onion plants cv. Giza Red. Foliar spray with growth stimulants were applied thrice at 45, 60 and75 days after transplanting. The design of the experiment was a complete randomized blocks with three replicates. Theresults showed that foliar spray of seaweeds extract at 4 or 5 g/l followed by yeast extract 4g/l had the higheststimulation effect on onion plant growth traits (plant height, number of leaves and leaf fresh weight) total bulb yield andits components as well as N, P and K content of bulb tissues. Application of seaweed extract (5 g/l) showed the leastweight loss (%) and decay (%) of bulbs compared to the control and other treatments.
https://hjsc.journals.ekb.eg/article_59099_65446038b4740a4f08a58eff00241240.pdf
2018-07-20
55
62
10.21608/hjsc.2018.59099
Allium cepa L
growth stimulants -growth-yield- storability
Ahmed, M.E.M
A. A.A. Sharaf
1
AUTHOR
ORIGINAL_ARTICLE
Effect of 1-MCP Treatment on Keeping Quality of Tomato Fruits at Ambient Temperature
The study was conducted during the spring 2017 and 2018 at the Horticulture Research Institute(A,R,C)in Giza, Egypt . Tomato fruits (Lycopersicum esculentum L.) obtained from a local field in Nawag village, Tanta city,Al-Gharbia Governorate, tomato were harvest at the Breaker-turning fruits stage, were chosen to the influence of 1-methylcyclopropene (1-MCP) concentration on retarding ripening process of green tomato fruit and maintainedquality, tomato fruit were fully immersed in aqueous 1-MCP at 50, 100, and 150μgL-1for 10 min, then fruit quicklydried, and then kept in 20 ◦C. Results indicated that 1-mcp treatment were retained quality fruit firmness of tomatoesfruit in comparison to control especially high concentration
https://hjsc.journals.ekb.eg/article_59100_6493a69ba6e49cd9e1e902648098a84d.pdf
2018-07-20
63
72
10.21608/hjsc.2018.59100
1-MCP
Tomato
fruit quality
Storage period
Ahmed, M. E. M
M.A.F. El-Naggar
1
AUTHOR
ORIGINAL_ARTICLE
Effect of Different Nitrogen Fertilizer Rates and Sources on Vegetative Growth and Yield of Quinoa Plant as a Newly Leafy Vegetable Crop
Quinoa (Chenopodium quinoa Willd.) is a pseudocereal that has been cultivated in the Andean region forthousands of years. It is considered as a new non-traditional leafy vegetable crop newly introduced to Egypt. Nitrogen ismore important than any other elements for plant growth. It plays a vital role in the growth, yield and nutritional qualityof leafy vegetable crops. In order to evaluate the influence of two sources of nitrogen (ammonium sulfate 20.6% andcalcium nitrate 15.5%) used at rates of 40, 60 and 80 kg N/fed. on vegetative growth and productivity of two quinoacultivars Cica and Hualhuas harvested after 40 days from sowing date. A field experiment was carried out at theExperimental Farm of the Horticulture Department, Faculty of Agriculture, Ain Shams University, Shoubra El-Kheima,Cairo, Egypt, during the two winter seasons of 2015 and 2016. The treatments were arranged in a split plot design, withthree replicates. The obtained results showed that Cica cultivar was superior to Hualhuas concerning all vegetativegrowth parameters and yield except for leaf area in both seasons of the study. Regarding nitrogen rates and sources,quinoa plants received 80 kg N/fed. from ammonium sulfate or calcium nitrate recorded the highest values of plantlength, leaf area, plant fresh and dry weights, and fresh and dry weights of leaves/plant, as well as leaf moisture contentwithout significant differences between the two nitrogen sources. On the other hand, the lowest values were recorded by40 kg N/fed. from ammonium sulfate or calcium nitrate except for number of leaves/plant in both seasons and leafmoisture content in the second season. However, no significant differences were detected among all nitrogen treatments(rates or sources) on the number of leaves/plant. A higher significant yield was attained by ammonium sulfate at 80 kgN/fed. in relative to the rest of nitrogen treatments. Concerning the interaction effect, the results clearly revealed thatCica plants received calcium nitrate at 80 kg N/fed. gave the highest values of plant length. Furthermore, Cica plantstreated with 80 kg N/ fed. either from ammonium sulfate or calcium nitrate recorded significant increases in plant freshand dry weights as well as yield without significant differences between them. On the other hand, the best values of leafarea were detected when Hualhuas plants were fertilized by 80 kg N/fed. from ammonium sulfate or calcium nitratewithout significant difference between them.
https://hjsc.journals.ekb.eg/article_59101_815196039e2726c1dab24455204db82a.pdf
2018-07-20
73
80
10.21608/hjsc.2018.59101
Chenopodium quinoa
N. rates and sources
Vegetative growth
yield
ORIGINAL_ARTICLE
Effect of Modified Atmosphere Packaging on Storability, Shelf Life and Quality of Snow Peas
This study aimed at selecting the most suitable package for snow peas, with maintaining the quality ofpods. Samples were packaged using six types of packages: control bag, non-perforated polypropylene, polypropylenewith 6, 12, 24 microholes and smart package. samples were then stored for 7, 14 and 21 days at 0˚C, with 90-95% R.H.and then kept sealed two and four additional days at 10˚C, with 80-85% R.H. to study the effect of modified atmospherepackaging on quality attributes, the atmosphere composition inside the packages and weight loss of snow peas. Resultsrevealed that microperforated polypropylene with 12 microholes bags are the suitable package to maintain the qualityduring storage and retail sale period, in terms of visual quality, taste, vitamin C, SSC, sugars content, reducing weightloss and prevent decay.
https://hjsc.journals.ekb.eg/article_59102_8f65a1b081edc004654625d4607f97d8.pdf
2018-07-20
81
90
10.21608/hjsc.2018.59102
Pisum sativum L. var. macrocarpon
MAP
polypropylene
smart package
retail sale
Nasef, I.N
M. W. M. Elwan
1
AUTHOR
ORIGINAL_ARTICLE
In Vitro Propagation of Nemaguard Peach (Prunus persica l.) Rootstock
This study was carried out at the Plant Tissue Culture Laboratory in Faculty of EnvironmentalAgricultural Sciences (FEAS), Arish University, Egypt during 2012 through 2015. The aim of this study was usingtissue culture technique for micropropagation of peach (Prunus persica L.) plant. Nodes and shoot tips were cultured onMS, NN, WPM and B5 media containing macro and micro elements as well as vitamins, supplemented with 100 mgl-1myo-inositol and 30 % sucrose. Shoot tip cultured on the MS medium had the best combination for the establishmentstage of mother plants. Multiple shoot tips were obtained on MS medium supplemented with 1.00 mgl-1 BA and 0.05mgl-1 NAA. The highest elongation of shoots was with 1.0 mgl-1 GA3.The highest rooting of shoots was with fullstrength MS medium with 2.00 mgl-1 IBA. Hardening the rooted shoots was done in a greenhouse in pots containingmixture of peatmoss, vermiculite and sand at the rate1:1:1. Plantlets were successfully acclimated with 93 % survive
https://hjsc.journals.ekb.eg/article_59116_ebb74b616af062eacd9848b3085f45c6.pdf
2018-09-01
91
98
10.21608/hjsc.2018.59116
Prunus persica L
Nemaguard rootstock
micropropagation
cytokinins
auxin
adenine sulphate
GA3
El-Deeb M.D
Shimaa M. I. Shaban
1
AUTHOR
ORIGINAL_ARTICLE
A comparative Study on the Propagation of Some Imported Peach Rootstocks by Using Hardwood Cuttings
The present investigation was conducted during two successive seasons (2015/2016 and2016/2017) to study the effects of hardwood cuttings collecting dates treated with indole-3-butyric acid (IBA), i.e. 0,200 (24 h soaking) and 2000 ppm (1 min. dipping) on %rooted cuttings, average number of roots/cutting, averagelength of roots/cutting (cm), average root length/cutting (cm) and % survival of rooted cuttings of peach [Prunuspersica (L.) Batsch] rootstocks viz. Cadaman, Nemaguard, Okinawa and Nemaredunder greenhouse conditions. Theeffect of cutting collection dates on cuttings total indoles, total phenols and total indoles/total phenols ratio, totalcarbohydrates, total nitrogen and C/N ratio, was also studied. The best date for collecting hardwood cuttings was thefirst of December since the mature shoots of the rootstocks contained the highest values of total indoles, total phenolsand total carbohydrates and the lowest value of total nitrogen. The rooting potential of cuttings was positively correlatedwith their content of total indoles, total phenols, total indoles/total phenols ratio, total carbohydrate and C:N ratio, whilehad negative correlation with their content of total nitrogen.The capacity of hardwood cuttings to develop adventitious roots varies among the rootstocks and between theIBA treatments. IBA at 2000 ppm recorded the highest values of % rooted cuttings (75.37 and 73.01%), and % survivalof rooted cuttings (74.83 and 69.38%) for Cadaman, while IBA at 200ppm was more effective in % rooted cuttings(69.18 and 64.39%), av. No. of roots/cutting (16.67 and 15.17), average length of roots/cutting (57.72 and 50.57cm), av.root length/cutting ( 3.48 and 3.41cm) and % survival of rooted cuttings (65.84 and 63.48%) for Okinawa (as the meanof two dates during the two seasons of study respectively). Nemaguard recorded the lowest values in % Rooted cuttingsand % Survival, while Nemared recorded moderate values in these respects. This study demonstrated the possibilitypropagation of imported peach rootstocks "Cadaman, Nemaguard, Okinawa and Nemared" locally by using hardwoodcuttings to limit import from abroad and save hard currency for the country
https://hjsc.journals.ekb.eg/article_59117_fa0a0388f808147dba0794aa8ee94950.pdf
2018-07-01
99
106
10.21608/hjsc.2018.59117
Peach
hardwood cutting
IBA
rooted cutting
Survival
Galal I. Eliwa
M. M. Wahba
1
AUTHOR
ORIGINAL_ARTICLE
Use of Biotechnology for Multiplication of Curcuma longa L. plant during six subcultures
The aim of this study was to set a protocol for the in vitro culture of Curcuma longa L. Sprouted budexplants of C. longa L. were explanted on Murashige and Skoog (MS) medium supplemented with differentconcentrations of 6-benzyladenine (BA) 0, 1, 3, 5, and 7 mg l-1 during six subcultures. The maximum number ofmultiplied shoots (8.60 and 8.70 shoots explant-1), was obtained in the medium containing 7 mg l−1 BA at 4th and5thsubculture with (90 and 100% multiplication frequency) respectively. The optimum BA concentrations for maximumnumber of multiplication varied across subcultures. The 7 mg l-1 BA concentration at the late subculture 4th, 5th and 6thproduce highest values of (number of shoots, longest shoot, number of leaves and fresh weight).The maximum numberof roots (6.38 roots explant-1) was induced from 3 mg l−1IBA. The tallest roots (40.6 and 36.5 cm) were obtained on MSmedium supplemented with 1 mg l−1 NAA and 3 mg l−1 IBA. Also, the heaviest fresh weight was obtained frommedium containing 2 and 3 mg l−1 IBA and all concentrations of NAA. In vitro plantlets immediately acclimatized togreenhouse conditions, showing 100% survival rates in a peat moss and vermiculite (1:3) medium.
https://hjsc.journals.ekb.eg/article_59119_dfdc7e8f15ec8879f69f6832d4813181.pdf
2018-07-01
113
119
10.21608/hjsc.2018.59119
in Vitro
Mass production
subculture
Koriesh, E. M
Hefni M. M.
1
AUTHOR
ORIGINAL_ARTICLE
Impact of Magnetized Water, Mineral Fertilization and Potassium Forms on Cowpea Growth and Chemical Constituents
The present work was conducted at the Experimental Station Farm, Faculty of Agriculture, MansouraUniversity, Egypt, during the two successive summer seasons of 2016 and 2017 to investigate the impact of irrigationwater (normal water - magnetized water), foliar spraying with K forms (potassium nitrate - potacrysal as a source ofnano potassium fertilizer) and levels of soil mineral fertilizers (100 -75 -50 %), NPK from recommended dose and theirinteractions on vegetative growth and chemical contents of cowpea plants.Obtained data cleared that irrigation with magnetized water produced the highest significant values of plantgrowth, chlorophylls and chemical composition contents compared to normal water treatments. Meanwhile, fertilizedcowpea plants with 100% NPK from recommended dose gave the maximum values of all studied parameters.Moreover, foliar spraying with potacrysal as a nono source of potassium increased significantly all studied characters,except number of branches in the second season.It could be recommended that irrigation cowpea plants with magnetized water, 75% NPK from therecommended dose and foliar spraying with potacrysal in order to limiting the environmental pollution and decreasecosts comparing to 100% NPK.
https://hjsc.journals.ekb.eg/article_59125_c3eb7f34218736dcb4ae987c5477a8ad.pdf
2018-09-01
1
6
10.21608/hjsc.2018.59125
NPK levels
Cowpea
potassium nitrate
Magnetic water
vegetative growth and chemical contents
Abd El-Hady
Samar M.A. Doklega
1
AUTHOR
ORIGINAL_ARTICLE
Multiplication and Regeneration Potential in Strawberry genotypes using Different In Vitro Culture Methods and Growth Regulators
In vitro experiment was conducted at the Plant Tissue Culture Laboratory of the Department ofHorticulture, Faculty of Agriculture, Suez Canal University, Ismailia, Egypt during the period 2015-2016. Effects ofincreasing number of subcultures up to 12th subculture, BA concentrations up to 2.0 mg/l and their interaction onmultiplication rate of four strawberry cultivars were studied. Also, organogenic potential of leaf disc explants fromthree strawberry cultivars were tested using two different media (direct and indirect regeneration medium). Presentedresults showed that the highest multiplication rate in cvs. Kamela and Amega were achieved after the 6th subculturesusing 1.0 mg/l BA, however, the highest multiplication rate in cv. Camarosa was achieved after 7 subcultures using 1.0mg/ BA. The highest multiplication rate in cv. Fortuna was found after 6 subcultures on MS medium+0.5 mg/l BA,however, the lowest multiplication rate in all tested genotypes were after 12th subculture on MS+ 2.0 mg/l BA. Highregeneration capacity was obtained in medium amended with TDZ (direct regeneration medium) compared with 2,4-D+BA (indirect regeneration medium). Fortuna gave the highest regenerated shoots followed by cv. Kamela, then cv.Camarosa (v/v/v). Plantlets were successfully acclimated with 90% survival.
https://hjsc.journals.ekb.eg/article_59126_13a7b0639ed6faec854473cfc2c57832.pdf
2018-07-01
47
54
10.21608/hjsc.2018.59126
Fragaria x ananassa
proliferation
Regeneration
genotypes
Mohamed, F.H.
M.A.A. Hussein
1
AUTHOR
ORIGINAL_ARTICLE
Effect of the Elicitor Hyaluronic acid on Multiplication and Indirect Regeneration of Curcuma longa L. Plant
The aim of this study was to evaluate the effect of hyaluronic acid (HA) concentrations for multiplicationand indirect regeneration of Curcuma longa L. plant. Multiplied shoots of C. longa L. were used as explant materialsand callus for indirect regeneration. The explants cultured on Murashige and Skoog (MS) medium supplemented withdifferent concentrations of HA as0.05, 0.5, 1.5, 3.0 and 15 mg l-1 for multiplication and 0.0, 0.16, 0.33, 0.66, 1.00, 2.00and 5.00 mg l−1 for indirect regeneration. Concerning to multiplication, the highest number of shoot (3.77 shootsexplant-1) was obtained from 3 mg l−1 HA. While, the least concentrations of HA (0.16, 0.33 and 0.66 mg l-1) enhancedshoot regeneration. On the other hand, the highest concentrations of HA produced higher number of roots and heaviestweight of callus under light condition.
https://hjsc.journals.ekb.eg/article_59127_834bee749819cd74e8e484fc1a1d4782.pdf
2018-07-01
107
112
10.21608/hjsc.2018.59127
Curcuma longa L
HA
Hyaluronic acid
multiplication
indirect regeneration
Turmeric
Koriesh, E. M.
M. M. Hefni
1
AUTHOR